kits for mmp2  (Elabscience Biotechnology)

Journal: International Journal of Molecular Medicine

Article Title: Bumetanide-blocked SLC12A2 exerts a protective effect in experimental diabetic retinopathy

doi: 10.3892/ijmm.2026.5774

Figure Lengend Snippet: Effect of SLC12A2 knockdown on inflammatory factor release and blood-retinal barrier in HG-treated rat retinal microvascular endothelial cells. (A) Levels of VEGF, IL-1β, IL-6 and TNF-α detected by ELISA. (B) Immunofluorescence staining showing the ZO-1 expression in each group. (C) Levels of MMP2 and MMP9 detected by commercial kits. Data from three independent experiments are indicated as the mean ± SD (n=3). * P<0.05 and ** P<0.01. HG, high glucose; VEGF, vascular endothelial growth factor; IL-1β, interleukin-1Beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha; ZO-1, zonula occludens-1; MMP, matrix metalloproteinase; n.s., not significant.

Article Snippet: In the present study, kits for MMP2 (cat. no. E-EL-R0618) and MMP9 (cat. no. E-EL-R3021; both from Elabscience Biotechnology, Inc.) were employed to detect the activity of these two factors in cell supernatants.

Techniques: Knockdown, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing

Journal: Cell Proliferation

Article Title: Soluble Sema4D From γδ T Cells Exerts Osteoblast Inhibition via Plexin‐B/ mTOR Signalling Contributing to Pathogenesis of Bisphosphonate‐Related Osteonecrosis of the Jaws

doi: 10.1111/cpr.70114

Figure Lengend Snippet: Autocrine MMP3 secretion induced mSema4D cleavage in γδ T cells. (A) KEGG analysis of DEGs in WT ZOL and TCRδ −/− ZOL mice was highly enriched in matrix metalloproteinase (MMP)‐related pathways. (B) The mRNA levels of Mmp3 and Adam17 in the extraction sockets of WT ZOL and TCRδ −/− ZOL mice. n = 4, * p < 0.05, ns indicates no significance. (C) The mRNA levels of Adam17 , Adam10 , Mmp2 , Mmp3 , Mmp9 and Mmp11 after γδ T cells were incubated with 15 μM ZOL for 3 and 6 days. Only the mRNA level of Mmp3 increased significantly and kept a high level after 6 days incubation. n = 6, * p < 0.05, ** p < 0.01. (D) The protein level of MMP3 was much higher than MMP2 and MMP9 in the γδ T cell supernatant measured by ELISA. n = 4, ** p < 0.01, ns indicates no significance. (E) The proportions of γδ T cells after treatment with GM6001 and TAPI‐2. GM6001 and TAPI‐2 are MMP inhibitors. T10 and T20 indicate 10 and 20 nM TAPI‐2, respectively; G50 and G100 indicate 50 and 100 nM GM6001, respectively. n = 6, ns indicates no significance. (F) The expression of Sema4D on γδ T cells after treatment with GM6001 and TAPI‐2. (G) Analysis of Sema4D expression in γδ T cells after treatment with GM6001 or TAPI‐2 on day 2 and 4. n = 6, * p < 0.05, ** p < 0.01. (H) The level of sSema4D in the supernatant of γδ T cells after treatment with GM6001 or TAPI‐2. n = 3, * p < 0.05, ** p < 0.01. (I) The level of sSema4D in the supernatant of γδ T cells after incubating with MMP2, MMP3 and MMP9 in synergy with GM6001. n = 4, * p < 0.05, ** p < 0.01. Data are mean ± SD.

Article Snippet: The kits of sSema4D (ELK5378, ELK Biotechnology), MMP2 (E‐EL‐M0780, Elabscience), MMP3 (E‐EL‐M0626) and MMP9 (E‐EL‐M3052) were used following the manufacturer's instructions.

Techniques: Extraction, Incubation, Enzyme-linked Immunosorbent Assay, Expressing